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dc.contributor.authorKumar, Pramod
dc.contributor.authorSatyam, Abhigyan
dc.contributor.authorFan, Xingliang
dc.contributor.authorCollin, Estelle
dc.contributor.authorRochev, Yury
dc.contributor.authorRodriguez, Brian J.
dc.contributor.authorGorelov, Alexander
dc.contributor.authorDillon, Simon
dc.contributor.authorJoshi, Lokesh
dc.contributor.authorRaghunath, Michael
dc.contributor.authorPandit, Abhay
dc.contributor.authorZeugolis, Dimitrios I.
dc.date.accessioned2018-09-20T16:13:43Z
dc.date.available2018-09-20T16:13:43Z
dc.date.issued2015-03-04
dc.identifier.citationKumar, Pramod; Satyam, Abhigyan; Fan, Xingliang; Collin, Estelle; Rochev, Yury; Rodriguez, Brian J. Gorelov, Alexander; Dillon, Simon; Joshi, Lokesh; Raghunath, Michael; Pandit, Abhay; Zeugolis, Dimitrios I. (2015). Macromolecularly crowded in vitro microenvironments accelerate the production of extracellular matrix-rich supramolecular assemblies. Scientific Reports 5 ,
dc.identifier.issn2045-2322
dc.identifier.urihttp://hdl.handle.net/10379/12337
dc.description.abstractTherapeutic strategies based on the principles of tissue engineering by self-assembly put forward the notion that functional regeneration can be achieved by utilising the inherent capacity of cells to create highly sophisticated supramolecular assemblies. However, in dilute ex vivo microenvironments, prolonged culture time is required to develop an extracellular matrix-rich implantable device. Herein, we assessed the influence of macromolecular crowding, a biophysical phenomenon that regulates intra- and extra-cellular activities in multicellular organisms, in human corneal fibroblast culture. In the presence of macromolecules, abundant extracellular matrix deposition was evidenced as fast as 48 h in culture, even at low serum concentration. Temperature responsive copolymers allowed the detachment of dense and cohesive supramolecularly assembled living substitutes within 6 days in culture. Morphological, histological, gene and protein analysis assays demonstrated maintenance of tissue-specific function. Macromolecular crowding opens new avenues for a more rational design in engineering of clinically relevant tissue modules in vitro.
dc.publisherSpringer Nature
dc.relation.ispartofScientific Reports
dc.subjectstem-cell culture
dc.subjectadult arterial revascularization
dc.subjecthuman corneal fibroblasts
dc.subjectgrowth-factor regulation
dc.subjectfetal bovine serum
dc.subjectkeratocyte phenotype
dc.subjectcollagen matrices
dc.subjectconditioned media
dc.subjectepithelial-cells
dc.subjectascorbic-acid
dc.titleMacromolecularly crowded in vitro microenvironments accelerate the production of extracellular matrix-rich supramolecular assemblies
dc.typeArticle
dc.identifier.doi10.1038/srep08729
dc.local.publishedsourcehttps://www.nature.com/articles/srep08729.pdf
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