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dc.contributor.authorMangan, Hazel
dc.contributor.authorGailín, Michael Ó
dc.contributor.authorMcStay, Brian
dc.date.accessioned2018-09-20T16:15:49Z
dc.date.available2018-09-20T16:15:49Z
dc.date.issued2017-06-02
dc.identifier.citationMangan, Hazel; Gailín, Michael Ó; McStay, Brian (2017). Integrating the genomic architecture of human nucleolar organizer regions with the biophysical properties of nucleoli. The FEBS Journal 284 (23), 3977-3985
dc.identifier.issn1742-464X
dc.identifier.urihttp://hdl.handle.net/10379/12622
dc.description.abstractNucleoli are the sites of ribosome biogenesis and the largest membraneless subnuclear structures. They are intimately linked with growth and proliferation control and function as sensors of cellular stress. Nucleoli form around arrays of ribosomal gene (rDNA) repeats also called nucleolar organizer regions (NORs). In humans, NORs are located on the short arms of all five human acrocentric chromosomes. Multiple NORs contribute to the formation of large heterochromatin-surrounded nucleoli observed in most human cells. Here we will review recent findings about their genomic architecture. The dynamic nature of nucleoli began to be appreciated with the advent of photodynamic experiments using fluorescent protein fusions. We review more recent data on nucleoli in Xenopus germinal vesicles (GVs) which has revealed a liquid droplet-like behavior that facilitates nucleolar fusion. Further analysis in both XenopusGVs and Drosophila embryos indicates that the internal organization of nucleoli is generated by a combination of liquid-liquid phase separation and active processes involving rDNA. We will attempt to integrate these recent findings with the genomic architecture of human NORs to advance our understanding of how nucleoli form and respond to stress in human cells.
dc.publisherWiley-Blackwell
dc.relation.ispartofThe FEBS Journal
dc.subjecthuman acrocentric chromosome
dc.subjectliquid droplet
dc.subjectliquid-liquid phase separation
dc.subjectnucleolar organizer region
dc.subjectnucleolus
dc.subjectribosomal gene
dc.subjectupstream binding factor
dc.subjectpolymerase-i transcription
dc.subjectearly xenopus-embryos
dc.subjectribosomal-rna genes
dc.subjecthuman-cells
dc.subjectacrocentric chromosomes
dc.subjectsynthetic nucleoli
dc.subjectubf
dc.subjectchromatin
dc.subjectrepeats
dc.subjectdamage
dc.titleIntegrating the genomic architecture of human nucleolar organizer regions with the biophysical properties of nucleoli
dc.typeArticle
dc.identifier.doi10.1111/febs.14108
dc.local.publishedsourcehttp://onlinelibrary.wiley.com/doi/10.1111/febs.14108/pdf
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