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dc.contributor.authorPerez, Ruth A.
dc.contributor.authorRehmann, Nils
dc.contributor.authorCrain, Sheila
dc.contributor.authorLeBlanc, Patricia
dc.contributor.authorCraft, Cheryl
dc.contributor.authorMacKinnon, Shawna
dc.contributor.authorReeves, Kelley
dc.contributor.authorBurton, Ian W.
dc.contributor.authorWalter, John A.
dc.contributor.authorHess, Philipp
dc.contributor.authorQuilliam, Michael A.
dc.contributor.authorMelanson, Jeremy E.
dc.date.accessioned2018-09-20T16:21:26Z
dc.date.available2018-09-20T16:21:26Z
dc.date.issued2010-09-09
dc.identifier.citationPerez, Ruth A. Rehmann, Nils; Crain, Sheila; LeBlanc, Patricia; Craft, Cheryl; MacKinnon, Shawna; Reeves, Kelley; Burton, Ian W.; Walter, John A.; Hess, Philipp; Quilliam, Michael A.; Melanson, Jeremy E. (2010). The preparation of certified calibration solutions for azaspiracid-1, -2, and -3, potent marine biotoxins found in shellfish. Analytical and Bioanalytical Chemistry 398 (5), 2243-2252
dc.identifier.issn1618-2642,1618-2650
dc.identifier.urihttp://hdl.handle.net/10379/13450
dc.description.abstractThe production and certification of a series of azaspiracid (AZA) calibration solution reference materials is described. Azaspiracids were isolated from contaminated mussels, purified by preparative liquid chromatography and dried under vacuum to the anhydrous form. The purity was assessed by liquid chromatography-mass spectrometry and nuclear magnetic resonance spectroscopy. The final concentration of each AZA in a CD(3)OH stock solution was determined by quantitative NMR spectroscopy. This solution was then diluted very accurately in degassed, high purity methanol to a concentration of 1.47 +/- 0.08 mu mol/L for CRM-AZA1, 1.52 +/- 0.05 mu mol/L for CRM-AZA2, and 1.37 +/- 0.13 mu mol/L for CRM-AZA3. Aliquots were dispensed into argon-filled glass ampoules, which were immediately flame-sealed. The calibration solutions are suitable for method development, method validation, calibration of liquid chromatography or mass spectrometry instrumentation and quality control of shellfish monitoring programs.
dc.publisherSpringer Nature
dc.relation.ispartofAnalytical and Bioanalytical Chemistry
dc.subjectreference materials
dc.subjectmass spectrometry/lc-ms
dc.subjectmarine toxins
dc.subjecttandem mass-spectrometry
dc.subjectmytilus-edulis
dc.subjecttoxins
dc.subjectmussels
dc.subjectstability
dc.subjectuncertainty
dc.subjectperformance
dc.subjectanalogs
dc.subjectcrms
dc.titleThe preparation of certified calibration solutions for azaspiracid-1, -2, and -3, potent marine biotoxins found in shellfish
dc.typeArticle
dc.identifier.doi10.1007/s00216-010-4161-2
dc.local.publishedsourcehttp://oar.marine.ie/bitstream/10793/444/1/The%20Preparation%20of%20Certified%20Calibration%20Solutions.pdf
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