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dc.contributor.authorKurbanoglu, Sevinc
dc.contributor.authorZafar, Muhammed Nadeem
dc.contributor.authorTasca, Federico
dc.contributor.authorAslam, Iqra
dc.contributor.authorSpadiut, Oliver
dc.contributor.authorLeech, Dónal
dc.contributor.authorHaltrich, Dietmar
dc.contributor.authorGorton, Lo
dc.date.accessioned2019-02-18T11:44:21Z
dc.date.issued2018-04-25
dc.identifier.citationKurbanoglu, Sevinc, Zafar, Muhammed Nadeem, Tasca, Federico, Aslam, Iqra, Spadiut, Oliver, Leech, Dónal Haltrich, Dietmar, Gorton, Lo. (2018). Amperometric Flow Injection Analysis of Glucose and Galactose Based on Engineered Pyranose 2-Oxidases and Osmium Polymers for Biosensor Applications. Electroanalysis, 30(7), 1496-1504. doi: doi:10.1002/elan.201800096en_IE
dc.identifier.issn1521-4109
dc.identifier.urihttp://hdl.handle.net/10379/14964
dc.description.abstractIn the present study, wild type and three mutants of pyranose 2-oxidase (PyOx-WT, PyOx-MT1, PyOx-MT2, PyOx-MT3), which showed improved properties for D-galactose oxidation, were investigated for their oxidising ability when immobilised on graphite electrodes. Four different flexible Os polymers with formal potentials ranging between -0.140 and 0.270V vs. Ag|AgCl0.1MKCl were applied together with the various forms of PyOx to wire graphite electrodes using polyethylene glycol diglycidyl ether as crosslinking reagent. The pH profiles for the electrodes modified with wild type and all PyOx mutants in combination with the Os polymers were investigated with both glucose and galactose, respectively, since the PyOx variants showed an improved catalytic activity for galactose. All modified electrodes showed highest response in the pH range between 8.5-10 and K-M, I-max values for both glucose and galactose were determined. To prove the catalytic activity, the biosensors were also characterized with cyclic voltammetry. A protein amount 0.26U was found optimum for PyOx-WT, 0.36U for PyOx-MT1, 0.41U for PyOx-MT2 and 0.28U for PyOx-MT3 and the analytical characterization of the enzyme electrodes was performed for glucose and galactose under optimized conditions.en_IE
dc.description.sponsorshipThe authors thank the following agencies for financial support: MN Zafar; The Higher Education Commission of Pakistan for financial support (grant number: 6515/Punjab/NRPU/R&D/HEC/2016), LG; The Swedish Research Council (grant number 2014‐5908), DL and LG; The European Commission (grant number: FP7‐PEOPLE‐2013‐ITN‐607793), and FT; The Fondecyt project 11130167 and the Proyecto Bsale 155.en_IE
dc.formatapplication/pdfen_IE
dc.language.isoenen_IE
dc.publisherWileyen_IE
dc.relation.ispartofElectroanalysisen
dc.subjectBiosensoren_IE
dc.subjectbiofuel cell anodeen_IE
dc.subjectpyranose oxidaseen_IE
dc.subjectosmium redox polymersen_IE
dc.subjectglucoseen_IE
dc.subjectgalactoseen_IE
dc.subjectGLASSY-CARBON ELECTRODESen_IE
dc.subjectMICROBIAL FUEL-CELLSen_IE
dc.subjectLESS BIOFUEL CELLen_IE
dc.subjectREDOX-POLYMERen_IE
dc.subjectCURRENT-DENSITYen_IE
dc.subjectCOULOMBIC EFFICIENCYen_IE
dc.subjectENZYME ELECTRODESen_IE
dc.subjectPHANEROCHAETE-CHRYSOSPORIUMen_IE
dc.subjectCELLOBIOSE DEHYDROGENASEen_IE
dc.subjectELECTRICAL CONNECTIONen_IE
dc.titleAmperometric flow injection analysis of glucose and galactose based on engineered pyranose 2-oxidases and osmium polymers for biosensor applicationsen_IE
dc.typeArticleen_IE
dc.date.updated2019-02-15T11:54:05Z
dc.identifier.doi10.1002/elan.201800096
dc.local.publishedsourcehttps://doi.org/10.1002/elan.201800096en_IE
dc.description.peer-reviewedpeer-reviewed
dc.contributor.funderSeventh Framework Programmeen_IE
dc.description.embargo2019-04-25
dc.internal.rssid14713396
dc.local.contactDonal Leech, School Of Chemistry, Room C228 Arts/Science Building, University Road, Nui Galway. 5349 Email: donal.leech@nuigalway.ie
dc.local.copyrightcheckedYes
dc.local.versionACCEPTED
dcterms.projectinfo:eu-repo/grantAgreement/EC/FP7::SP3::PEOPLE/607793/EU/Biofuel Cells : From fundamentals to applications of bioelectrochemistry/BIOENERGYen_IE
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