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dc.contributor.authorO’Brien, Killian P.
dc.contributor.authorRamphul, Eimear
dc.contributor.authorHoward, Linda
dc.contributor.authorGallagher, William M.
dc.contributor.authorMalone, Carmel
dc.contributor.authorKerin, Michael J.
dc.contributor.authorDwyer, Róisín M.
dc.identifier.citationO’Brien K.P. et al. (2017) Circulating MicroRNAs in Cancer. In: Rani S. (eds) MicroRNA Profiling. Methods in Molecular Biology, vol 1509. Humana Press, New York, NYen_IE
dc.description.abstractIt is believed that microRNAs have potential as circulating biomarkers of disease, however successful clinical implementation remains a challenge. This chapter highlights broad variations in approaches to microRNA analysis where whole blood, serum and plasma have each been employed as viable sources. Further discrepancies in approaches are seen in endogenous controls and extraction methods utilised. This has resulted in contradictory publications, even when the same microRNA is targeted in the same disease setting. Analysis of blood samples highlighted the impact of both collection method and storage, on the microRNA profile. Analysis of a panel of microRNAs across whole blood, serum and plasma originating from the same individual emphasised the impact of starting material on microRNA profile. This is a highly topical field of research with immense potential for translation into the clinical setting. Standardisation of sample harvesting, processing and analysis will be key to this translation. Methods of sample harvesting, preservation and analysis are outlined, with important mitigating factors highlighted.en_IE
dc.description.sponsorshipThis material is based upon works supported by the Irish Cancer Society collaborative cancer research centre BREAST-PREDICT Grant CCRC13GAL and funding agency Breast Cancer Research.en_IE
dc.publisherHumana Pressen_IE
dc.relation.ispartofMicroRNA Profilingen
dc.subjectCirculating microRNAsen_IE
dc.subjectBreast Canceren_IE
dc.subjectWhole blooden_IE
dc.titleCirculating MicroRNAs in canceren_IE
dc.typeBook chapteren_IE
dc.description.peer-reviewedPeer reviewed
dc.contributor.funderIrish Cancer Societyen_IE
dc.local.contactRóisín Dwyer, Surgery, Nui Galway. 3008 Email:

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