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dc.contributor.authorAlexandrov, Sergey
dc.contributor.authorMcGrath, James
dc.contributor.authorSheppard, Colin J. R.
dc.contributor.authorBoccafoschi, Francesca
dc.contributor.authorGiannini, Cinzia
dc.contributor.authorSibillano, Teresa
dc.contributor.authorSubhash, Hrebesh
dc.contributor.authorHogan, Josh
dc.contributor.authorLeahy, James J.
dc.identifier.citationAlexandrov, Sergey A. , McGrath, James , Sheppard, Colin , Boccafoschi, Francesca, Giannini, Cinzia, Sibillano, Teresa, Subhash, Hrebesh, Hogan, Josh, Leahy, James J. (2019). Ultra-sensitive label free imaging below the resolution limit (Conference Presentation) (Proceedings Vol. 10891). Paper presented at the SPIE BiOS, 2019, San Francisco, United States, 02-07 February.en_IE
dc.description.abstractAlmost all known nanoscopy methods rely upon the contrast created by fluorescent labels attached to the object of interest. This causes limitations on their applicability to in vivo imaging. A new label-free spectral encoding of spatial frequency (SESF) approach to nanoscale probing of three-dimensional structures has been developed. It was demonstrated that spatial frequencies, encoded with optical wavelengths, can be passed though the optical system independently of the resolution of the imaging system. As a result information about small size structures can be detected even using a low resolution imaging system. Different versions of the SESF imaging have been published [1-7], including a novel contrast mechanism for high resolution imaging [1], real time nano-sensitive imaging [2], reconstruction the axial (along depth) spatial frequency profiles for each point with nano-sensitivity to structural changes [3], and the adaptation of the SESF approach to depth resolved imaging [4,5]. Recently the SESF approach has been applied to overcome the diffraction limit and dramatically improve resolution [6,7]. Here we present further development of the SESF approach including correlation mapping SESF imaging. Both results of numerical simulation and preliminary experimental results, including biological samples, will be presented. [1] Alexandrov,, Opt. Lett. 36 3323 (2011). [2] Alexandrov,, Opt. Express 20 (8) 9203 (2012). [3] Alexandrov,, Appl. Phys. Let., 101 033702 (2012). [4] Uttam,, Opt. Express, 21, 7488 (2013). [5] Alexandrov,, Nanoscale, 6, 3545 (2014). [6] Alexandrov,, Sci. Rep., 5, doi: 10.1038/srep13274 (2015). [7] Alexandrov,, J. Biophotonics, (2018).en_IE
dc.description.sponsorshipThis project has received funding from the European Union’s Horizon 2020 research and innovation programme under grant agreement No 761214. The materials presented and views expressed here are the responsibility of the author(s) only. The EU Commission takes no responsibility for any use made of the information set out.en_IE
dc.publisherSPIE (Society of Photo-optical Instrumentation Engineers) Digital Libraryen_IE
dc.relation.ispartofSPIE Photonics West 2019, BiOS, San Francisco, USAen
dc.subjectImage resolutionen_IE
dc.subjectReal time imagingen_IE
dc.subjectSpatial frequenciesen_IE
dc.subjectImaging systemsen_IE
dc.subjectSpatial resolutionen_IE
dc.subjectComputer programmingen_IE
dc.subjectFluorescent markersen_IE
dc.subjectIn vivo imagingen_IE
dc.subjectSuper resolution microscopyen_IE
dc.titleUltra-sensitive label free imaging below the resolution limiten_IE
dc.typeConference Paperen_IE
dc.contributor.funderHorizon 2020en_IE
dc.local.contactSergey Alexandrov, School Of Physics, Nui Galway. - Email:
dcterms.projectinfo:eu-repo/grantAgreement/EC/H2020::RIA/761214/EU/NanoSTARS imaging for STEM cell therapy for arthritic joints/STARSTEMen_IE

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