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dc.contributor.authorKumar, Pramod
dc.contributor.authorSatyam, Abhigyan
dc.contributor.authorFan, Xingliang
dc.contributor.authorCollin, Estelle
dc.contributor.authorRochev, Yury
dc.contributor.authorJoshi, Lokesh
dc.contributor.authorPandit, Abhay
dc.contributor.authorZeugolis, Dimitrios I.
dc.identifier.citationKumar P, Satyam A, Fan X, Collin E, Rochev Y, Rodriguez BJ, Gorelov A, Dillon S, Joshi L, Raghunath M, Pandit A, Zeugolis DI (2015) 'Macromolecularly crowded in vitro microenvironments accelerate the production of extracellular matrix-rich supramolecular assemblies'. Scientific reports, 5 .en_IE
dc.descriptionJournal articleen_IE
dc.description.abstractTherapeutic strategies based on the principles of tissue engineering by self-assembly put forward the notion that functional regeneration can be achieved by utilising the inherent capacity of cells to create highly sophisticated supramolecular assemblies. However, in dilute ex vivo microenvironments, prolonged culture time is required to develop an extracellular matrix-rich implantable device. Herein, we assessed the influence of macromolecular crowding, a biophysical phenomenon that regulates intra- and extra-cellular activities in multicellular organisms, in human corneal fibroblast culture. In the presence of macromolecules, abundant extracellular matrix deposition was evidenced as fast as 48 h in culture, even at low serum concentration. Temperature responsive copolymers allowed the detachment of dense and cohesive supramolecularly assembled living substitutes within 6 days in culture. Morphological, histological, gene and protein analysis assays demonstrated maintenance of tissue-specific function. Macromolecular crowding opens new avenues for a more rational design in engineering of clinically relevant tissue modules in vitro.en_IE
dc.description.sponsorshipScience Foundation Ireland, Research Frontiers Programme, Project Number: SFI-09-RFP-ENM2483en_IE
dc.publisherNature Publishing Groupen_IE
dc.relation.ispartofScientific reportsen
dc.subjectTissue engineeringen_IE
dc.subjectIn vitroen_IE
dc.subjectMacromolecular crowdingen_IE
dc.titleMacromolecularly crowded in vitro microenvironments accelerate the production of extracellular matrix-rich supramolecular assemblies.en_IE
dc.local.contactAbhay Shashikant Pandit, Curam, Biosciences, Nui Galway. 2758 Email:

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